About this manual. This manual describes the operation of the ÄKTA™FPLC™ system. System description, system maintenance and trouble-shooting are also. filter. Sample pump. Buffer select valve (V6). Injection valve (V1). Reverse . Manual. Flowpath. B1 or B2. BufferValveB. Pump A. Pump B. Common inlet. A2. A1. About this manual This manual describes the operation of ÄKTA purifier: Evaluating the UV response Calculate the UV response ratios in the following.
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Attach the column holder enclosed in Box above the UV cell.
Do not tighten TeflonTM tubing further as this will damage the end of the tubing. Follow the recommendations in this chapter to keep the system in good working order. The description is common for all purofier systems, unless otherwise clearly stated in the contents. Then click OK to acknowledge the printer chosen. Check the valves are operating correctly. The latter function is based on peak detection using slope or level sensing. In case of an emergency situation, the system mains switch must always be easy to access and the mains plug must always be easy to disconnect.
Allow the straps to remain taped to the plastic cover. Refer to the “Technical specifications” for fuse data. Double-click a result file icon in the list to the right. Fraction collector installed optional. Those marked Excluded will not be run.
Click here to select page Run setup consists of a number of pages. For analytical reproducibility, use a sample volume that is 5 times the volume of the sample loop. The minimum flow rate for BufferPrep is amta. The modules and components contain high voltage circuits that can give a lethal electric shock. Unstable conductivity curve 7. When a new method is created and a recipe is selected, default correction mannual will apply.
The log is saved in the result file. A copy of these terms and conditions is available on request. Select a position that minimizes the distance between the column and the optical unit. In case of an emergency situation, the system mains switch must always be easy to access, and the mains plug must always be easy to disconnect.
Click the xaxis to view the method in time, volume or column volumes. Help us improve your experience by sending an error report. Some of the pre-programmed recipes have default correction factors. The information selected here will be printed in the report.
The Method Wizard covers the most commonly used chromatographic techniques. Only purifidr parts that are approved or supplied by GE Healthcare may be used for maintaining or servicing the system.
Check that there is no air left in the inlet tubing.
Installation description, system description, system maintenance and troubleshooting are also found in this manual. Pump and are explained in Table The method run is initiated in a series of pages in Run Setup in System Control. Run Setup in Method Editor. Cables, capillaries, accessories, column holder, etc.
ÄKTApurifier User Guide – GE Healthcare Life Sciences
Remove the column and the pH electrode optional. These valves can be used to accomplish the following functions: Only use mains cables delivered or approved by GE Healthcare. Only one mains inlet is required. Layouts can be selected in Save current layout and all your saved selections will apply.
Monitor UPC consists of a control unit, an optical unit with lamp assembly and a choice of two flow cells optical path length 2 mm or 5 mma conductivity flow cell with integrated temperature sensor and a pH flow cell with pH electrode optional.
ÄKTApurifier – GE Healthcare Life Sciences
A 5 mm cell is also included in the package. Pull back the plastic cover 3 to uncover the swivel platform 4 and raise the system to an upright position. This is described later in this Section. Contact your local GE Healthcare representative for the most current information. The curves are named as Result Partial filling is achieved as follows: Change channel plate and distribution plate.
Peak fractionation is selected on the Elution Fractionation page in the Method Wizard.
Set the valve manually to this port. Pressure reading Once a year or when required. Follow the step-by-step instructions below and check the “Installation Record. Then, when running the method, use a piece of tubing to replace the column. Aktz complete filling, the sample volume can only be changed by changing the loop size.
The first one is active as long as the pressure is below the set point.